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KMID : 0382420030290020080
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Korean Journal of Environmental Health Society
2003 Volume.29 No. 2 p.80 ~ p.86
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Effect of Cyclohexane Treatment on Serum Level of Glutathione S-Transferase Activity in Liver Damaged Rats
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Oh Jeong-Dae
Yoon Chong-Guk
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Abstract
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To evaluate the effect of cyclohexane(CH) treatment on the serum levels of glutathion S-transferase(GST) activity in liver damaged animals, damaged liver was induced with pretreatment of 50% CCI., dissolved in olive oil (0.1 mlllOOg body weight) intraperitoneally 17 times every other day. To CCl₄-treated rats, CH (1.56 g/kg body weight. i.p) was injected once and then the animals were sacrificed at 4 hours after injection of CH. The CCl₄-treated animals were identified as severe liver damage on the basis of liver functional findings, i,e, increased serum levels of alanine aminotransferase(ALT), alkaline phosphate(ALP) and xanthine oxidase(XO) activities. On the other hand, CCl₄-treated animals injected with CH once(CCl₄-pretreated animals) showed more decreased serum levels of ALT and XO, and more increased those of ALP mther than CCl₄-treated animals. In case of comparing the GST with ALT activity in liver, both CCl₄-treated and pretreated animals showed similar changing pattern of enzyme actvity. Especially CCl₄-pretreated animals showed significantly increased serum level of GST actvity compared with the CCl₄-treated those, whereas those of ALT showed reversed tendency. In aspects of GST enzyme kinetics, CCl₄-pretreated animals showed higher Vmax of liver GST enzyme than CCl₄-treated animals. In conclusion. injection of CH to the liver damaged rats led to enhanced liver damage and more increased activity of serum GST which may be chiefly caused by the enzyme induction.
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